BRL 3A大鼠肝細胞
描述：這個細胞株的無血清條件培養基是MSA的一個來源。 MSA多肽家族能夠部分滿足雞胚成纖維細胞對血清的需求。
動物種別：大鼠
性別：***
組織來源：肝
形態：***
培養基和添加劑：DMEM培養基(GIBCO,貨號12800017，添加NaHCO3 1.5g/L)，90%；胎牛血清，10%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。
供應限制：A。僅供研究之用。
REFERENCE：Nissley SP , et al. Proliferation of buffalo rat liver cells in serum-free medium does not depend upon multiplication-stimulating activity (MSA). Cell 11: 441-446, 1977. PubMed: 302146 Coon HG , Weiss MC . A quantitative comparison of formation of spontaneous and virus- produced viable hybrids. Proc. Natl. Acad. Sci. USA 62: 852-859, 1969. PubMed: 4308097 Dulak NC , Temin HM . A partially purified polypeptide fraction from rat liver cell conditioned medium with multiplication-stimulating activity for embryo fibroblasts. J. Cell. Physiol. 81: 153-170, 1973. PubMed: 4735141 Dulak NC , Shing YW . Large scale purification and further characterization of a rat liver cell conditioned medium multiplication stimulating activity. J. Cell. Physiol. www.atcc360.com。90: 127-130, 1977. PubMed: 833209 Schalch DS , et al. Nonsuppressible insulin-like activity (NSILA). I. Development of a new sensitive competitive protein-binding assay for determination of serum levels. J. Clin. Endocrinol.
中文名稱：大鼠肝細胞
