小鼠胚胎細胞NIH/3T3
| ??【編號】：NIH/3T3 ??【名稱】：小鼠胚胎細胞NIH/3T3 ??【規格】：株 ??【價格】：1800   小鼠胚胎細胞
細胞名稱：NIH/3T3小鼠胚胎細胞
描述：與原始的隨機交配3T3和近交系BALB/c 3T3建株方法一樣，NIH/3T3，是從NIH Swiss小鼠胚胎培養物中建立的高度接觸抑制的連續細胞株。 為了培育在形態學特征上更適合于進行轉化分析的亞株，建立的NIH/3T3細胞株又進行了五輪以上亞克隆。 這株細胞對DNA轉化及轉染研究十分有用。 檢測表明肢骨發育畸形病毒(鼠痘)陰性。
動物種別：小鼠
性別：不明
組織來源：胚胎
形態：成纖維細胞
培養基和添加劑：DMEM培養基(GIBCO,貨號12800017，添加NaHCO3 1.5g/L)，90%；優質胎牛血清，10%。 氣相：空氣，95%；二氧化碳，5%。 溫度：37攝氏度。
供應限制：A。僅供研究之用。
REFERENCE：Jainchill JL , et al. Murine sarcoma and leukemia viruses: assay using clonal lines of contact-inhibited mouse cells. J. Virol. 4: 549-553, 1969. PubMed: 4311790 Andersson P , et al. A defined subgenomic fragment of in vitro synthesized Moloney sarcoma virus DNA can induce cell transformation upon transfection. Cell 16: 63-75, 1979. PubMed: 84715 Copeland NG , Cooper GM . Transfection by exogenous and endogenous murine retrovirus DNAs. Cell 16: 347-356, 1979. PubMed: 222457 Loffler S , et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321 Berson JF , et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 8709256 Jones PL , et al. Tumor necrosis factor alpha and interleukin-1beta regulate the murine manganese superoxide dismutase gene through a complex intronic enhancer involving C/EBP-beta and NF-kappaB. Mol. Cell. Biol. 17: 6970-6981, 1997. PubMed: 9372929 Gonzalez Armas JC , et al. DNA immunization confers protection against murine cytomegalovirus infection. J. Virol. 70: 7921-7928, 1996. PubMed: 8892915 Siess DC , et al. Exceptional fusogenicity of chinese hamster ovary cells with murine retrovirus suggests roles for cellular factor(s) and receptor clusters in the membrane fusion process. J. Virol. 70: 3432-439, 1996. PubMed: 8648675 Jang SI , et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649 Medin JA , et al. Correction in trans for Fabry disease: expression, secretion, and uptake of alpha-galactosidase A in patient-derived cells driven by a high-titer recombinant retroviral vector. Proc. Natl. Acad. Sci. USA 93: 7917-7922, 1996. PubMed: 8755577 Lee JH , et al. The proximal promoter of the human transglutaminase 3 gene. J. Biol. Chem. 271: 4561-4568, 1996. PubMed: 8626812 Chang K , Pastan I . Molecular cloning of mesothelin, a differentiation antigen present on mesothelium, mesotheliomas, and ovarian cancers. Proc. Natl. Acad. Sci. USA 93: 136-140, 1996. PubMed: 8552591 Cranmer LD , et al. Identification, analysis, and evolutionary relationships of the putative murine cytomegalovirus homologs of the human cytomegalovirus UL82 (pp71) and UL83 (pp65) matrix phosphoproteins. J. Virol. 70: 7929-7939, 1996. PubMed: 8892916 Shisler J , et al. Induction of susceptibility to tumor necrosis factor by E1A is dependent on binding to either p300 or p105-Rb and induction of DNA synthesis. J. Virol. 70: 68-77, 1996. PubMed: 8523594 Cavanaugh VJ , et al. Murine cytomegalovirus with a deletion of genes spanning HindIII-J and -I displays altered cell and tissue tropism. J. Virol. 70: 1365-1374, 1996. PubMed: 8627652 Westerman KA , Leboulch P . Reversible immortalization of mammalian cells mediated by retroviral transfer and site-specific recombination. Proc. Natl. Acad. Sci. USA 93: 8971-8976, 1996. PubMed: 8799138 Tumor 22(1):5-8,2002 Chin J Cell Mol Immunol 18(2):146-148, 2002 Chin J Immunol 18(1):33-35,2002 Acta Biologiae Experimentalis Sinica 36(1):5-12,2003 J Fujian Med Univ 37(2):144-146,2003 Shanghai Immunol Journal 23(1):16-19,2003
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