α-糜蛋白酶 來源于牛胰腺(純化), ≥45 units/mg protein
Product Name： α-Chymotrypsin from bovine pancreas(Purified)
別名：α-胰凝乳蛋白酶
CAS號：9004-07-3
介紹： Chymotrypsin preferentially catalyzes the hydrolysis of peptide bonds involving L-isomers of tyrosine, phenylalanine and tryptophan. It also readily acts upon amides and esters of susceptible amino acids. Chymotrypsin catalyzes the hydrolysis of bonds of leucyl, methionyl, asparaginyl and glutamyl residues.www.atcc360.com
貯存： 儲存溫度2-8℃
敏感性： 易潮濕
生化和生理學機理： 一種絲氨酸蛋白酶，能將與芳香烴或其他大型疏水性側鏈（Tyr、Trp、Phe、Met、Leu）結合的肽從鍵的羧基端水解。 α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. Its pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu2+ and Hg2+.
