Escherichia coli
DSM 116065
BACTERIA
How to read the following data (Example)
Name:
Escherichia coli(Migula 1895) Castellani and Chalmers 1919
DSM No.:
116065
Strain designation:
A19 Stablelabel
Isolated from:
laboratory techniques
Country:
Germany
Date of sampling:
01.02.2022
Nagoya Protocol Restrictions:
There are NO known Nagoya Protocol restrictions for this strain.
History:
<- R. Levin and F. Bernhard, Univ. Frankfurt, Biocentre; A19 Stablelabel
Cultivation conditions:
Medium 381, strain is auxotrophic for glutamine, 37°C
add 1 g/L glutamine to medium
Complete DSMZ Media List
Summary and
additional information:
<- R. Levin and F. Bernhard, Univ. Frankfurt, Biocentre; A19 Stablelabel. Laboratory techniques. Germany. Derivative of strain K-12 A19 useful for optimized cell-free synthesis with reduced amino acid scrambling activity for highly productive lysates to produce labeled proteins for NMR studies, with deleted genes involved in amino acid metabolism. TheE. coliA19 Stablelabel S30 lysate has cumulated mutations and shows decreased metabolic activity on amino acids L-asp, L-asn, L-glu and L-gln. The following genes are deleted with residual cloning scars of FRT site left after marker recovery: asnA, ansA, ansB, aspC, ilvE, glnA. Strain depositors could demonstrate optimized NMR spectral complexity of selectively labeled proteins CF-synthesized in Stablelabel lysates, strain is useful for synthetic biology and protein engineering. Always requires glutamine in medium. (Medium 381, 37°C). add 1 g/L glutamine to medium
Risk group:
1 (classification according to GermanTRBA)
Restrictions:
German law regulating genetic engineering / Gentechnikgesetz),Category C
